论文部分内容阅读
目的:观察罗格列酮对兔主动脉斑块基质金属蛋白酶(MMP)-2、基质金属蛋白酶抑制因子(TIMP)-2表达的影响。方法:健康纯系新西兰大白兔27只,随机分为对照组、模型组、罗格列酮组。实验10周末,酶法检测血脂水平,取主动脉弓标本,切片行苏木素-伊红(HE)染色,并采用免疫组织化学法检测兔主动脉粥样硬化斑块内MMP-2、TIMP-2的表达,并观察主动脉斑块形态学变化。结果:与模型组比较,罗格列酮组动脉粥样硬化斑块和泡沫细胞数减少,MMP-2表达显著降低,而TIMP-2表达显著增加(P<0.05)。结论:罗格列酮可通过下调兔主动脉粥样硬化组织中MMP-2及上调TIMP-2的表达,减轻大动脉内膜的炎症反应以及调节免疫等机制而发挥抗动脉粥样硬化作用。
Objective: To observe the effect of rosiglitazone on the expression of matrix metalloproteinase (MMP) -2 and matrix metalloproteinase inhibitor (TIMP) -2 in aortic plaque in rabbits. Methods: A total of 27 healthy New Zealand white rabbits were randomly divided into control group, model group and rosiglitazone group. At the end of the experiment, serum lipids levels were measured by enzyme-linked immunosorbent assay (ELISA). Aortic arch specimens were obtained and stained with hematoxylin and eosin (HE). Immunohistochemistry was used to detect the expression of MMP-2 and TIMP-2 in rabbit aortic atherosclerotic plaques , And observed aortic plaque morphology changes. Results: Compared with the model group, the number of atherosclerotic plaque and foam cells in rosiglitazone group decreased, while the expression of MMP-2 decreased significantly, while the expression of TIMP-2 increased significantly (P <0.05). CONCLUSION: Rosiglitazone exerts anti-atherosclerotic effects by down-regulating the expression of MMP-2 and up-regulating TIMP-2 in rabbit aortic atherosclerosis, attenuating inflammation in the intima of the aorta and regulating immunity.