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目的含有组织抗体的内耳病患者血清与未固定及未脱钙的仓鼠头部冰冻切片反应可避免抗原性的改变,而且在同一张切片上耳蜗以外的组织可以用来对照。方法66份前庭听觉功能障碍或听力正常的患者血清和一份正常人的血清与仓鼠头部切片进行反应,结果抗血管内皮抗体、抗核抗体以及抗线粒体抗体阳性的血清和仓鼠头部切片上的内耳以及其它组织呈现阳性反应。表明患者血清中的这些抗体对内耳来说不是器官特异性的。50例前庭听觉功能障碍的患者血清中只有2例有耳硬化的患者血清在免疫发病机理上可以看作是半器官特异性的。抗内膜抗体和抗平滑肌抗体阳性的血清对内耳组织反应阴性。对头皮肌和镫骨动脉壁及内耳动脉壁有反应。Corti氏器的盖膜显示出非特异性的自身绿色荧光。结论头部切片对于区分器官特异性抗体以及诊断自身免疫性内耳病是一种新的、方便的且安全的技术。单纯的免疫组化测定并没有对内耳疾病的单因素病因学给出一个提示。在使用患者血清中的组织抗体时,其它的额外因素也应该被考虑在内,因为前庭听觉功能障碍在病因学可能是多因素的。
Purpose The serum of patients with internal ear disease containing tissue antibodies reacted with frozen sections of hamsters’ heads not fixed and not decalcified to avoid changes in antigenicity, and tissues other than the cochlea on the same slice could be used as a control. Methods 66 patients with vestibular dysfunction or hearing normal serum and normal human serum reacted with the hamster head section. The results of anti-vascular endothelial antibody, anti-nuclear antibody and anti-mitochondrial antibody-positive serum and hamster head section The inner ear and other tissues showed a positive reaction. Indicating that these antibodies in the patient’s serum are not organ-specific to the inner ear. Serum from 50 patients with auditory dysfunction of the vestibular arteries has only 2 cases of otosclerosis sera that can be considered semi-organ specific on the immunopathogenesis. Anti-intima and anti-smooth muscle antibody-positive sera negative for inner ear tissue. The scalp muscle and tarsal artery wall and the inner ear artery wall reaction. Corti’s cap membrane shows non-specific self-green fluorescence. Conclusion Head biopsy is a new, convenient and safe technique for distinguishing organ-specific antibodies and diagnosing autoimmune inner ear disease. A simple immunohistochemical assay did not give a clue to the single-factor etiology of the inner ear disease. Other additional factors should also be considered when using tissue antibodies in patient serum, as auditory dysfunction in the vestibular space may be multifactorial in etiology.