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为探讨可控条件下实现大蒜有性生殖的可能性,1996～2004年,从国内外搜集的160份大蒜种质中筛选出41份抽薹早、总苞发育好的品系,于现薹后8～10d(打弯期)切取带茎花总苞,剔除营养芽(气生鳞茎),置温度(25±2)℃,日光照13～16h,相对湿度75%的培养室液培促进开花,延长开花时间,花期最长达76d。混合花粉人工授粉426朵花,其中319朵(78.6%)子房明显膨大。从11份子房、胚珠发育好的品系中选膨大的子房固定,石蜡切片,用普通光镜观察到完整的有性胚结构;选240个膨大子房接种在B5+蔗糖4%～12%培养基上,置培养室内培养47～56d,53个子房继续膨大,保持绿色,形成不同发育阶段的胚,其中34个子房发育出76个完整胚。将完整胚接种到MS+BA0.01～0.05mg/L+NAA0.05～0.10mg/L+蔗糖40～80g/L培养基上,置同上条件的培养室内培养42～47d,发育成有性生殖的完整小植株。
In order to explore the possibility of sexual reproduction of garlic under controlled conditions, from 1996 to 2004, 41 germplasm with early bolls and well-developed buds were screened from 160 garlic germplasms collected at home and abroad. (10 ± 1) ~ 10d (bending period), the buds with stems were excised and the vegetative buds (aerial bulbs) were removed. The liquid culture in the culture chamber with the relative humidity of 75% To extend the flowering time, flowering up to 76d. Mixed pollen artificial pollination of 426 flowers, of which 319 (78.6%) ovarian significantly inflated. Expand the ovary from 11 ovary and ovule-developed strains, fix the paraffin sections and observe the complete sexual embryo structure with ordinary light microscope. Select 240 expanded ovary cells in 4% ~ 12% medium of B5 + sucrose On the other hand, cultured in vitro for 47-56 days, the 53 ovary continued to enlarge and remained green, forming embryos at different developmental stages. Among them, 76 embryos were developed in 34 ovary. The whole embryos were inoculated into MS + BA0.01 ~ 0.05mg / L + NAA0.05 ~ 0.10mg / L + sucrose 40 ~ 80g / L medium and incubated for 42 ~ 47d in the same condition, Complete plantlets.