论文部分内容阅读
目的:建立HPLC法测定花锚草及乙肝健片中1-羟基3,4,5-三甲氧基口山酮含量的方法。方法:采用HPLC法,使用HypersilC18柱(5μm,4.6mm×250mm),以甲醇-0.5%的磷酸(60∶40)为流动相,流速为1.0ml/min-1,检测波长243nm。结果:1-羟基3,4,5-三甲氧基口山酮在0.01416μg~0.1062μg,(r=0.9999)范围内与峰面积呈良好的线性关系,平均回收率为98.3%,RSD=2.07%(n=6)。结论:本法简便、快速、结果满意,可作为花锚草及乙肝健片质量控制的依据。
Objective: To establish a HPLC method for the determination of 1-hydroxy-3,4,5-trimethoxyoxyproterone in Huagancao and Hepatitis B tablets. Methods: HPLC method using Hypersil C18 column (5μm, 4.6mm × 250mm), methanol - 0.5% phosphoric acid (60:40) as the mobile phase, flow rate 1.0ml/min-1, detection wavelength 243nm. RESULTS: 1-hydroxy 3,4,5-trimethoxy-orthosterone showed a good linear relationship with the peak area in the range of 0.01416 μg to 0.1062 μg (r=0.9999). The average recovery was 98.3%. RSD was 2.07. % (n=6). Conclusion: This method is simple, rapid and satisfactory. It can be used as the basis for quality control of Huagancao and Hepatitis B tablets.