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冠菌素可以提高棉花根系还原氯化三苯四唑(TTC)生成三苯甲臜(TF)的能力,本文研究了不同钾水平下冠菌素处理后的TF值在不同提取方法之间的差异及其与根系多酚和呼吸速率之间的关系。结果显示,TF热提取法的测定值明显高于常温提取法,尤其是冠菌素处理条件下。冠菌素处理后TF的OD485值明显高于OD520值,和冠菌素显著提高根系中花青素含量具有一致性。冠菌素处理显著提高了煮沸根和烘干根的TTC还原量,与显著提高根系多酚含量具有一致性。冠菌素显著提高了新鲜根系的TTC还原量,如其单位质量鲜根的OD520值分别是高钾和低钾的5.4和5.9倍(热提取法)或6.1和4.1倍(常温提取法),同时显著提高了根系多酚含量及单位质量的呼吸速率,这表明TTC还原能力可以用于表示冠菌素对根系代谢活性的促进,而不受多酚的影响。
Coronatine could improve the ability of cotton roots to reduce the production of trichlorethylene (TF) by triphenyltetrazolium chloride (TTC). In this paper, the TF values of coronatine treated with different potassium levels were compared among different extraction methods Differences and Their Relationship with Root Polyphenols and Respiration Rates. The results showed that TF thermal extraction method was significantly higher than the measured value of the extraction method at room temperature, especially coronatine treatment conditions. The OD485 of TF after coronatine treatment was significantly higher than that of OD520, which was in agreement with that of coronatine. Coronatine treatment significantly increased the TTC reduction of boiled and dried roots, which was in agreement with the significant increase of root polyphenol content. Coronatine significantly increased the TTC reduction of fresh roots. For example, the OD520 values of fresh roots per unit mass were 5.4 and 5.9 times (hot extraction) or 6.1 and 4.1 times (extraction at room temperature) of high potassium and low potassium, respectively Significantly increased root polyphenol content and unit mass respiration rate, suggesting that TTC reducing ability can be used to indicate that coronatine promotes root metabolic activity independent of polyphenols.