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目的:在前期研究的基础上,进一步研究榄香烯调控CDK8-P21通路逆转肺癌化疗耐药的机制。方法:复制小鼠Lewis肺癌模型,造模成功后随机分为四组:模型组、香烯组、多柔比星组及榄+多组,分别予生理盐水、榄香烯、多柔比星及二者联合用药7 d,取瘤组织,荧光定量PCR及Western Blot检测瘤组织中CDK8及P21基因、蛋白表达情况。结果:与模型组比较,多柔比星组肿瘤组织中CDK8及P21基因、蛋白表达均增加(P<0.05)。与榄香烯组比较,榄+多组中P21基因、蛋白表达增加(P<0.05),多柔比星组明显增加(P<0.01)。而CDK8基因、蛋白表达无明显差别(P>0.05)。结论:化疗药物会诱导CDK8-P21通路的激活;榄香烯通过抑制P21基因、蛋白表达逆转移肺癌化疗耐药,而与CDK8-P21通路无关。
OBJECTIVE: To further study the mechanism of elemene modulating CDK8-P21 pathway in reversing chemoresistance of lung cancer based on previous studies. Methods: The Lewis lung carcinoma model was duplicated in mice and then randomly divided into four groups: model group, celecoxib, doxorubicin group and olive + multiple groups, which were given normal saline, elemene, doxorubicin And the combination of both for 7 days, the tumor tissue, fluorescence quantitative PCR and Western Blot were used to detect the expression of CDK8 and P21 gene and protein. Results: Compared with the model group, the expression of CDK8 and P21 gene and protein in the doxorubicin group increased (P <0.05). Compared with elemene group, the expression of P21 gene increased in both groups (P <0.05) and in doxorubicin group (P <0.01). The CDK8 gene, protein expression no significant difference (P> 0.05). Conclusion: Chemotherapy can induce the activation of CDK8-P21 pathway. Elemene can reverse the chemoresistance of lung cancer by inhibiting the expression of P21 protein, but not with the CDK8-P21 pathway.