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作者应用显微镜观察曼氏血吸虫童虫和虫卵在体内移行的动态,试图阐明一些有争论的问题。给雌性小鼠皮下注射270、2,000、3,000或4,500条尾蚴,观察童虫的移行;用感染50或200条尾蚴的小鼠观察成虫或虫卵的移行。先将小鼠麻醉,气管插管给氧使肺充气,打开胸腔,置于显微镜台,暴露脏器,以37.5℃等渗液灌洗,覆以透明塑料薄膜,用380～710毫微米波长的单色光透照器官,放大65～625倍,用高分辨能力的光导摄象管电视系统控制亮度和对比度,用电视监控电影摄片记录图象。显微镜检前先肉眼检查。
The authors used microscopy to observe the dynamics of Schistosoma mansoni’s squirrels and insects migrating in vivo in an attempt to elucidate some of the controversial issues. Female mice were subcutaneously injected with 270, 2,000, 3,000, or 4,500 cercariae to observe the migratory behavior of the juveniles; the migration of adult or ovum was observed in mice infected with 50 or 200 cercariae. The mice were anesthetized and the endotracheal tube was oxygenated to inflate the lungs. The chest cavity was opened and placed on a microscope stage to expose organs. The cells were lavaged with an isotonic solution of 37.5 ° C and covered with a transparent plastic film. The cells were irradiated with a wavelength of 380-710 nm Monochromatic light transillumination organs, enlarged 65 ~ 625 times, with high resolution light guide tube television system brightness and contrast control, with a television camera to record images. Before the microscopic examination of the naked eye examination.