论文部分内容阅读
目的探讨选择性环氧合酶(COX-2)抑制剂塞来昔布对胃癌细胞株BGC823多药耐药(MDR)1表达的影响。方法 BGC823细胞株经不同浓度的塞来昔布处理后,用ELISA法检测胃癌细胞前列腺素E2(PGE2)的分泌;24,48h后用RT-PCR检测多药耐药MDR1mRNA的表达,48h后用免疫细胞化学染色法检测P-gp的表达。结果塞来昔布可显著抑制胃癌细胞株BGC823的PGE2分泌,并呈浓度依赖性(P<0.05)。不同浓度塞来昔布作用于细胞后,胃癌细胞株BGC823的MDR/P-gp表达受不同程度抑制,100μmol/L的塞来昔布对MDRlmRNA表达抑制作用强于10μmol/L(P<0.01)。作用48h与24h相比,塞来昔布对MDRlmRNA表达的抑制作用更强(P<0.01)。结论塞来昔布可抑制BGC823MDR1/P-gp的表达,且呈量效关系。其可能通过抑制COX-2活性而抑制PGE2表达,最终抑制P-gp的表达。
Objective To investigate the effect of selective cyclooxygenase (COX-2) inhibitor celecoxib on multidrug resistance (MDR) 1 in gastric cancer cell line BGC823. Methods BGC823 cells were treated with different concentrations of celecoxib and the secretion of prostaglandin E2 (PGE2) was detected by ELISA. The expression of multidrug-resistant MDR1 mRNA was detected by RT-PCR 24 and 48 hours after treatment with Immunocytochemical staining was used to detect the expression of P-gp. Results Celecoxib significantly inhibited PGE2 secretion in gastric cancer cell line BGC823 in a concentration-dependent manner (P <0.05). After treated with different concentrations of celecoxib, MDR / P-gp expression in gastric cancer cell line BGC823 was inhibited to some extent. The inhibition of MDR1 mRNA expression by 100μmol / L celecoxib was stronger than that of 10μmol / L (P <0.01) . The effect of celecoxib on MDR1 mRNA expression was stronger than that of 24h (P <0.01). Conclusion Celecoxib can inhibit the expression of BGC823MDR1 / P-gp in a dose-response manner. It may inhibit PGE2 expression by inhibiting COX-2 activity and eventually inhibit the expression of P-gp.