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目的:检测非小细胞肺癌(non smal celllungcancer,NSCLC)外周血和骨髓中粘蛋 白1(mucin1,MUC1)mRNA的表达。方法:应用 RT PCR技术,对31例肺癌患者、10例肺良性病 变患者和8名健康人外周血及骨髓中MUC1基 因mRNA表达进行检测。结果:31例肺癌患者 中10例检测到外周血,7例检测到骨髓中有 MUC1mRNA表达,10例肺良性病变患者和8名 健康人中未检测到MUC1mRNA表达。肺癌患 者外周血及骨髓中MUC1mRNA表达分别为 32.3%(10/31)和22.6%(7/31),两者之间存在 正相关关系,P<0.05;外周血和骨髓中MUC1 mRNA的表达与肺癌组织学类型、细胞分化程度 及pTNM分期均密切相关,P<0.05。结论:肺癌 患者外周血和骨髓中存在常规方法检测不出的 肿瘤细胞;应用巢式RT PCR法可检测到肺癌患 者外周血和骨髓中MUC1mRNA表达,可为制 定治疗方案和评估预后提供重要参考依据。
Objective: To detect the expression of mucin1 (MUC1) mRNA in peripheral blood and bone marrow of non-small cell lung cancer (NSCLC). Methods: RT-PCR was used to detect the mRNA expression of MUC1 in peripheral blood and bone marrow from 31 patients with lung cancer, 10 patients with benign lung disease and 8 healthy people. RESULTS: Peripheral blood was detected in 10 of 31 patients with lung cancer, MUC1 mRNA was detected in 7 of the bone marrow samples, and no MUC1 mRNA was detected in 10 patients with benign lung disease and 8 healthy controls. The expression of MUC1 mRNA in peripheral blood and bone marrow of patients with lung cancer was 32.3% (10/31) and 22.6% (7/31) respectively, there was a positive correlation between them Lung cancer histological types, cell differentiation and pTNM stage were closely related, P <0.05. CONCLUSIONS: There are tumor cells that can not be detected by routine methods in peripheral blood and bone marrow of patients with lung cancer. The expression of MUC1mRNA in peripheral blood and bone marrow of patients with lung cancer can be detected by nested RT-PCR, which may provide important reference for the formulation of treatment plan and evaluation of prognosis .