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目的研究贝科能(复合辅酶)对心肺复苏后大鼠心、脑、肾细胞超微结构是否具有保护作用及其机制。方法采用窒息合并冰氯化钾停跳液(0.5molL)致大鼠心跳骤停5min后开始心肺复苏的动物模型,SD大鼠24只,随机分为对照组(假手术组)、常规复苏组和贝科能治疗组,每组8只。复苏后24h采取组织标本,采用比色法测定心、脑、肾组织中丙二醛(MDA)含量及超氧化物歧化酶(SOD)、Na+K+ATPase活力,采用透射电镜观察心、脑、肾细胞超微结构。结果常规复苏组心、脑、肾组织中MDA含量明显增高(P<0.05),SOD及Na+K+ATPase活力显著降低(P<0.05);贝科能治疗组各项指标检测均比常规复苏组好。透射电镜下可见对照组大鼠心、脑、肾细胞超微结构形态正常,常规复苏组各器官细胞线粒体肿胀、嵴断裂、空泡变性等超微结构损伤性改变,贝科能治疗组细胞超微结构改变较轻。结论贝科能对心肺复苏后大鼠心、脑、肾细胞超微结构具有保护作用。
Objective To study whether beclomethasone (CoA) has a protective effect on the ultrastructure of heart, brain and kidney in rats after cardiopulmonary resuscitation (CPR) and its mechanism. Methods 24-SD rats were randomly divided into control group (sham-operated group), conventional resuscitation group And Beco therapy group, 8 in each group. Twenty-four hours after resuscitation, the tissue samples were taken and the contents of malondialdehyde (MDA), superoxide dismutase (SOD) and Na + K + ATPase in heart, brain and kidney tissues were measured by colorimetric method. Renal cell ultrastructure. Results The levels of MDA in heart, brain and kidney were significantly increased (P <0.05) and the activities of SOD and Na + K + ATPase in normal resuscitation group were significantly lower than those in normal resuscitation group (P <0.05) Good group. Under the TEM, the ultrastructure of heart, brain and kidney cells in control group were normal, and ultrastructural changes of mitochondria such as swelling, cristae and vacuolar degeneration were observed in all organs of conventional resuscitation group. Microstructure changes lighter. Conclusion Beco can protect the ultrastructure of heart, brain and kidney in rats after cardiopulmonary resuscitation.