论文部分内容阅读
目的了解辽宁省东港市2014年甲型肝炎(甲肝)病毒(Hepatitis A Virus,HAV)流行株基因型特征,为HAV溯源研究奠定基础。方法收集东港市部分甲肝病人血清标本,HAV结构-非结构区基因VP1-2A引物,经核酸提取,逆转录-聚合酶链反应(Reverse Transcription-Polymerase Chain Reaction,RT-PCR),序列测定,对HAV基因进行序列同源性及亲缘关系分析。结果本次检测到的东港市HAV都属于1A亚型,核苷酸序列同源性为98.1%~100%,与中国河北省、日本及韩国的流行株VP1-2A编码区基因有相同序列或同源性较高。结论东港市有多株HAV存在,流行可能有多个传染链,在人群免疫水平较低时,引起甲肝流行。
Objective To understand the genotypes of 2014 HAV epidemic strains in Donggang, Liaoning Province, and to lay a foundation for the study of HAV traceability. Methods Serum samples from HAV patients and VP1-2A HAV structural-non-structural domain genes were collected from Donggang city. The nucleic acid extraction, Reverse Transcription-Polymerase Chain Reaction (RT-PCR) HAV gene sequence homology and genetic relationship analysis. Results The HAVs in Donggang City were all of 1A subtype and the nucleotide sequence homology was 98.1% -100%. The HAV sequences shared the same sequence with the VP1-2A coding region of popular strains in Hebei, Japan and South Korea, or High homology. Conclusion There are many strains of HAV in Donggang City. There may be multiple infectious lines in the epidemic. When the immunization level of the population is low, it causes the epidemic of HAV.