A novel saline-tolerant bacterium Bacillus circulans WZ-12 was evaluated for its potential to degrade four chlorinated hydrocarbons under saline conditions.CH2Cl2 was effectively degraded by Bacillus circulans WZ-12 cells in the medium containing NaCl concentrations ranging from 5 g·L-1 to 10 g·L-1,and the maximum degradation efficiency(85%) was achieved at NaCl concentration of 10 g·L-1.Similarly,Bacillus circulans WZ-12 was able to degrade CH2 BrCl,C2H4Cl2,and C2H2Cl2 in the presence of 10 g NaCl per liter within 24 h.Cells of Bacillus circulans WZ-12 grown in minimal salt medium contained low levels of glycine betaine(GB),but GB levels were 3-to 5-fold higher in cells grown in media with high salt.Kinetic analysis revealed that biodegradation of the four chlorinated hydrocarbons was concentration dependent and a linear inverse correlation(R 2-0.85-0.94) was observed between the rate of biodegradation(V) and salt concentration from 5 g·L-1 to 60 g·L-1.The growing cells(in minimal salt medium) degraded approximately 50% of the CH2Cl2 within 24 h,whereas the resting cells(in physiological saline) degraded only 25% of the CH2Cl2 within 24 h and were inactive after 36 h cultivation.Biodegradation could be repeatedly performed for more than 192 h with more than 50% removal efficiency.Bacillus circulans WZ-12 grows well in an aqueous/oil system,hence,it is effective for the treatment of industrial effluents that contain chlorinated hydrocarbons with high salt concentrations. A novel saline-tolerant bacterium Bacillus circulans WZ-12 was evaluated for its potential to degrade four chlorinated hydrocarbons under saline conditions. CH2Cl2 was effectively degraded by Bacillus circulans WZ-12 cells in the medium containing NaCl concentrations ranging from 5 g · L-1 to 10 g · L-1, and the maximum degradation efficiency (85%) was achieved at NaCl concentration of 10 g · L-1.Similarly, Bacillus circulans WZ-12 was able to degrade CH2 BrCl, C2H4Cl2, and C2H2Cl2 in the Presence of 10 g NaCl per liter within 24 h. Cells of Bacillus circulans WZ-12 grown in minimal salt medium contained low levels of glycine betaine (GB), but GB levels were 3-to 5-fold higher in cells grown in media with high salt. Kinetic analysis revealed that biodegradation of the four chlorinated hydrocarbons was concentration dependent and a linear inverse correlation (R 2-0.85-0.94) was observed between the rate of biodegradation (V) and salt concentration from 5 g · L-1 to 60 g · L-1.The growing cells (in minima while the resting cells (in physiological saline) degraded only 25% of the CH2Cl2 within 24 h and were inactive after 36 h cultivation.Biodegradation could be more performed than more than 192 h with more than 50% removal efficiency. Bacillus circulans WZ-12 grows well in an aqueous / oil system, hence, it is effective for the treatment of industrial effluents that contain chlorinated hydrocarbons with high salt concentrations.