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为了观察小鼠睾丸生后发育过程中生殖细胞自发死亡的方式与规律。用生后０、１、４、７、１０、１３、１８和５０ｄＡ／Ｊ系小鼠，一侧睾丸用２．５％戊二醛，另一侧睾丸用４％多聚甲醛固定１８ｈ后，分别用树脂或石蜡包埋，制备超薄和连续石蜡切片，进行电镜观察和ＴＵＮＥＬ染色光镜观察。电镜观察显示，在出生当日，可见个别呈坏死特征的变性生殖细胞，核质溶解，但无染色质聚集。核周间隙扩大，线粒体和内质网等细胞器肿胀，有空泡形成。细胞膜有突起、出泡现象。生后１ｄ，死亡细胞罕见。生后４ｄ开始见到典型凋亡特征的生殖细胞，并于生后１０～１３ｄ达到高峰。ＴＵＮＥＬ染色见阳性细胞在生后４ｄ开始出现，１０～１３ｄ达高峰，而后逐渐减少，与电镜观察结果一致。结果表明：小鼠睾丸在发育过程中生殖细胞的自发死亡主要以凋亡的形式发生，在一定时期，则以坏死的方式出现。凋亡的发生与细胞的分裂增殖具有密切关系
In order to observe the way and rule of spontaneous death of germ cells during the development of mouse testes. At 0, 1, 4, 7, 10, 13, 18 and 50 dA / J mice, 2.5% glutaraldehyde was applied on one testis and 18% Respectively with resin or paraffin embedded, prepared thin and continuous paraffin sections, electron microscopy and TUNEL staining. Electron microscopy showed that on the day of birth, some degenerative germ cells characterized by necrosis were seen, with nucleolytic dissolution but no chromatin aggregation. Perinuclear space expansion, mitochondria and endoplasmic reticulum and other organelles swelling, there is empty bubble formation. Cell membrane protruding, a bubble phenomenon. 1d after birth, rare death cells. Germ cells with typical apoptotic features began to be seen 4 days after birth and peaked at 10 to 13 days after birth. TUNEL staining showed that positive cells began to appear 4 days after birth, peaked 10 ~ 13d, and then gradually decreased, consistent with the results of electron microscopy. The results showed that the spontaneous death of germ cells during the development of mouse testis occurs mainly in the form of apoptosis, and in a certain period of time, it occurs in a necrotic manner. Apoptosis occurs with the cell division and proliferation are closely related