论文部分内容阅读
目的 探讨尿毒症患者血浆同型半胱氨酸 (Hcy)的临床意义及其相关因素。方法 分别采用快速高压液相微柱层析法和Immlite全自动化学发光免疫分析仪测定 32例正常人和 32例尿毒症患者血液透析前后的血浆总Hcy和叶酸、维生素B12 浓度。结果 血液透析后血浆Hcy与叶酸较血液透析前明显下降 (P<0 .0 1) ,前者显著高于正常人 (P <0 .0 1) ,后者与正常值比较 ,均无显著差异 (P >0 .0 5 ) ;血液透析前叶酸与Hcy存在明显负相关 ,血浆维生素B12 与Hcy无显著相关性。内瘘堵塞组与非堵塞组比较 :血浆Hcy明显升高(P <0 .0 1) ;叶酸显著降低 (P <0 .0 5 )。结论 所有血液透析患者均存在高Hcy血症 ,可能是血液透析患者内瘘栓塞的危险因素之一。高Hcy血症的形成可能与叶酸相对缺乏有关
Objective To investigate the clinical significance of plasma homocysteine (Hcy) in uremia patients and its related factors. Methods Plasma total Hcy, folic acid and vitamin B12 concentrations in 32 normal subjects and 32 uremic patients before and after hemodialysis were determined by rapid high pressure liquid microcolumn microdialysis and Immlite automatic chemiluminescence immunoassay. Results After hemodialysis, plasma Hcy and folic acid were significantly lower than those before hemodialysis (P <0.01), the former was significantly higher than that of normal people (P <0.01), while the latter was not significantly different from the normal value P> 0.05). There was a significant negative correlation between folic acid and Hcy before hemodialysis. There was no significant correlation between plasma vitamin B12 and Hcy. Fistula occlusion group and non-occlusion group: plasma Hcy was significantly higher (P <0.01); folic acid was significantly lower (P <0.05). Conclusions All patients with hemodialysis have hyperhomocysteinemia, which may be one of the risk factors of fistula embolism in hemodialysis patients. Hyperhomocysteinemia may be related to the relative lack of folic acid