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目的探讨轮状病毒(rotavirus,RV)肠炎患儿血清和粪便上清液白细胞介素(interleukin,IL)-10、IL-1β、IL-19、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)的水平变化及临床意义。方法选择2015年4月—2016年4月住院治疗的RV肠炎患儿150例,根据1998年全国腹泻病防治学术研究会议制定的标准分为轻症组80例、重症组70例。同期选择体检健康儿童80例作为对照组。RV肠炎患儿均给予RV肠炎常规治疗7 d。治疗前后,采用化学发光法测定血清和粪便上清液标本IL-10、IL-19、IL-1β及TNF-α水平。计量资料多组比较采用方差分析,计量资料两两比较采用LSD-t检验。P<0.05为差异有统计学意义。结果治疗前,重症组与轻症组血清及粪便上清液IL-1β[(83.3±8.4)、(64.5±15.5)pg/ml,(46.7±6.8)、(38.7±12.6)pg/ml]、TNF-α水平[(62.3±16.6)、(54.5±14.5)pg/ml,(48.9±13.4)、(39.7±12.6)pg/ml]均高于对照组(均P<0.05),IL-10、IL-19水平[(12.3±6.6)、(10.3±5.4)pg/ml,(11.7±4.3)、(9.1±7.5)pg/ml,(18.8±7.4)、(17.1±6.2)pg/ml,(13.5±5.6)、(11.1±7.6)pg/ml]低于对照组(均P<0.05);重症组以上指标与轻症组比较差异有统计学意义(均P<0.05)。治疗后,重症组与轻症组血清及粪便上清液IL-1β、TNF-α、IL-10、IL-19水平与对照组比较差异无统计学意义(均P>0.05)。结论 RV肠炎患儿血清和粪便上清液IL-1β、TNF-α水平升高,IL-10、IL-19水平降低;对其进行检测可评估患儿的病情程度及治疗效果。
Objective To investigate the effects of interleukin (IL) -10, IL-1β, IL-19 and tumor necrosis factor-α (TNF-α) in serum and feces supernatant of children with rotavirus TNF-α) levels and clinical significance. Methods 150 children with RV enteritis treated in hospital from April 2015 to April 2016 were divided into 80 cases in mild disease group and 70 severe disease group according to the standard set by National Conference on Prevention and Treatment of Diarrhea in 1998. In the same period, 80 healthy children were selected as the control group. Children with RV enteritis were given conventional treatment of RV enteritis for 7 days. Before and after treatment, the levels of IL-10, IL-19, IL-1β and TNF-α in serum and feces supernatant were measured by chemiluminescence. Measurement data were compared using multiple groups analysis of variance, measurement data pairs comparison using LSD-t test. P <0.05 for the difference was statistically significant. Results Before treatment, the levels of IL-1β in the serum and feces supernatant in the severe and mild groups were (83.3 ± 8.4), (64.5 ± 15.5) pg / ml, (46.7 ± 6.8) and (38.7 ± 12.6) pg / (P <0.05). The level of TNF-α was significantly higher than that of the control group (62.3 ± 16.6 and 54.5 ± 14.5 pg / ml, 48.9 ± 13.4 and 39.7 ± 12.6 pg / ml, respectively) 10, IL-19 levels of (12.3 ± 6.6), (10.3 ± 5.4) pg / ml, 11.7 ± 4.3, 9.1 ± 7.5 pg / ml, 18.8 ± 7.4, 17.1 ± 6.2 pg / ml, (13.5 ± 5.6), (11.1 ± 7.6) pg / ml] in the severe group were significantly lower than those in the control group (all P <0.05). After treatment, the levels of IL-1β, TNF-α, IL-10 and IL-19 in serum and feces supernatant in severe group and mild group were not significantly different from those in control group (all P> 0.05). Conclusions The levels of IL-1β and TNF-α in serum and feces supernatant of children with RV enteritis are increased, and the levels of IL-10 and IL-19 are decreased. The severity of the disease and the therapeutic effect can be evaluated in children with RV enteritis.