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This paper first demonstrates second-harmonic generation(SHG) in the intact cell nucleus,which acts as an optical indicator of DNA malignancy in prostate glandular epithelial cells.Within a scanning region of 2.7 μm×2.7 μm in cell nuclei,SHG signals produced from benign prostatic hyperplasia(BPH) and prostate carcinoma(PC) tissues(mouse model C57BL/6) have been investigated.Statistical analyses(t test) of a total of 405 measurements(204 nuclei from BPH and 201 nuclei from PC) show that SHG signals from BPH and PC have a distinct difference(p < 0.05),suggesting a potential optical method of revealing very early malignancy in prostate glandular epithelial cells based upon induced biochemical and/or biophysical modifications in DNA.
This paper first demonstrates second-harmonic generation (SHG) in the intact cell nucleus, which acts as an optical indicator of DNA malignancy in prostate glandular epithelial cells. Winning a scanning region of 2.7 μm × 2.7 μm in cell nuclei, SHG signals produced from benign prostatic hyperplasia (BPH) and prostate carcinoma (PC) tissues (mouse model C57BL / 6) have been investigated. Statistical analyzes (t test) of a total of 405 measurements (204 nuclei from BPH and 201 nuclei from PC) show that SHG suggesting a potential optical method of revealing very early malignancy in prostate glandular epithelial cells based upon induced biochemical and / or biophysical modifications in DNA.