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通过萃取、正相硅胶、Sephadex LH-20、闪式柱色谱以及反相HPLC柱色谱等多种分离方法相结合,从信宜润楠乙醇提物中首次分离得到21个化合物;借助红外、质谱和核磁共振等波谱学分析方法鉴定了它们的结构,其中包括8个丁内酯类(1～8),8个木脂素类(9～16)和5个萜类化合物(17～21),化合物16是降七碳木脂烷类新天然产物。经体外活性筛选发现化合物5对胃癌(BGC-823)和卵巢癌(A2780)人肿瘤细胞株有选择性抑制活性,IC50分别为0.13×10-6,2.66×10-6mol.L-1;在1×10-5mol.L-1时,化合物8和9具有明显抑制PAF刺激大鼠多形核白细胞β-葡萄糖苷酸酶释放作用,抑制率分别为60.0%,54.2%。
Twenty-one compounds were isolated from the ethanol extract of Pleurotus cornucopiae by extraction, normal phase silica gel, Sephadex LH-20, flash column chromatography and reverse phase HPLC column chromatography. Their structures were elucidated by NMR and other spectroscopic methods, including 8 butyrolactones (1 ~ 8), 8 lignans (9 ~ 16) and 5 terpenoids (17 ~ 21) , Compound 16 is a new natural product of Heptaceryl Alkanes. In vitro activity screening showed that compound 5 had selective inhibitory activity against gastric cancer (BGC-823) and ovarian cancer (A2780) human cancer cell lines with IC50 of 0.13 × 10-6 and 2.66 × 10-6 mol·L-1, respectively. Compound 8 and 9 could significantly inhibit the release of β-glucuronidase from PAF-stimulated rat polymorphonuclear leukocytes with inhibition rates of 60.0% and 54.2% at 1 × 10-5 mol·L-1, respectively.