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为了解霉酚酸酯 (MMF )联合环孢素A (CsA )治疗移植物抗宿主病 (GVHD )后T淋巴细胞亚群的变化。我们采用免疫荧光标记和流式细胞仪技术 ,分析了 6例GVHD患者的T淋巴细胞亚群以及活化T细胞标志。发现在MMF治疗后患者的CD3+ CD4+ T细胞明显降低 ,CD3+ CD8+ T细胞明显增高 ,CD4/CD8比值下降 ;CD2 5 + CD4+ 和CD6 9+ CD8+ T细胞均升高。结果初步显示 ,MMF可能主要作用于CD3+ CD4+ T细胞 ,并与CsA在治疗GVHD方面有协同作用。
To understand the mycophenolate mofetil (MMF) combined with cyclosporin A (CsA) in the treatment of graft-versus-host disease (GVHD) changes in T lymphocyte subsets. We used immunofluorescent labeling and flow cytometry to analyze T-lymphocyte subsets and activated T-cell markers in 6 GVHD patients. It was found that the CD3 + CD4 + T cells were significantly decreased, the CD3 + CD8 + T cells were significantly increased and the ratio of CD4 / CD8 was decreased after MMF treatment. Both CD2 + CD4 + and CD6 9+ CD8 + T cells were increased. The results showed that MMF may mainly act on CD3 + CD4 + T cells and synergistic effect with CsA in the treatment of GVHD.