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本文报道了担子菌菌种24属43种122株经过液氮超低温冻结后的结果。122株担子菌分别用5％或10％二甲亚砜(DMSO)和10％甘油作保护剂,经快速和慢速冻结其成活率分别是,以甘油作保护剂,快速冻结为81％;慢速冻结为94％;以 DMSO 作保护剂时,快速冻结为97％;慢速冻结为100％。14属23种41株担子菌经冻结保存不同时间后,进行结菇试验,除银耳(Tremella fucifor-mis)的对照和冻结样品均未形成子实体外,其余属种均保持着结菇这一特性。
This paper reports the results of the suspension of 43 122 species of 24 genera of Basidiomycetes after cryogenic freezing in liquid nitrogen. 122 strains of basidiomycetes were protected by 5% or 10% DMSO and 10% glycerol, respectively. The survival rates of the 122 basidiomycetes were fast and slow freezing, respectively, with glycerol as the protective agent, which was rapidly frozen to 81%. Slow freezing to 94%; with DMSO as a protective agent, rapid freezing was 97%; slow freezing to 100%. Fourteen strains belonging to 41 species, belonging to 23 species, were frozen and stored at different times. After the mushrooms were inoculated for the same period, the test of mushrooms was carried out. All the species except the control and frozen samples of Tremella fucifor-mis did not form fruiting bodies. characteristic.