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长期紫外线照射会引发诸多皮肤相关疾病,皮肤的保养越来越受到学者们的广泛关注。本文研究组方提取物A对大鼠抗光老化的影响。通过病理学分析和电镜检测照射部位皮肤组织病理学形态及超微结构;采用免疫组化和荧光定量PCR检测皮肤基质金属蛋白酶1(MMP-1)、MMP-2、MMP-9和Ia抗原的表达以及皮肤组织中I型胶原、III型胶原、结缔组织生长因子(CTGF)、转化生长因子β1(TGF-β1)基因表达情况。研究表明:与模型对照组相比,组方提取物A低、中、高剂量组照射部位皮肤真皮间质血管扩张、炎细胞浸润、成纤维细胞增生减轻,真皮弹力纤维变性、局灶性蓄积减轻,皮肤表皮厚度、真皮层成纤维细胞数显著降低,照射部位皮肤的超微结构得到改善,提取物A可降低皮肤转化生长因子β1(TGF-β1)的表达,提高皮肤III型胶原的表达。因此,组方提取物A具有较好的抗光老化功效。
Long-term ultraviolet radiation will cause many skin-related diseases, skin care more and more scholars have been widespread concern. This study was to study the effects of Compound A on the anti-photo-aging in rats. The histopathology and ultrastructure of the skin at irradiation site were detected by pathological analysis and electron microscopy. The expressions of MMP-1, MMP-2, MMP-9 and Ia antigen were detected by immunohistochemistry and real-time PCR The expressions of type I collagen, type III collagen, connective tissue growth factor (CTGF) and transforming growth factor β1 (TGF-β1) in skin tissue were detected. The results showed that: compared with the model control group, the extract of the group A low, medium and high doses of radiation dermal dermal dilation, inflammatory cell infiltration, fibroblast proliferation, dermal elastic fibrosis, focal accumulation Reduce the thickness of the skin epidermis, significantly reduce the number of dermal fibroblasts, the ultrastructure of the skin irradiation site was improved, extract A can reduce the expression of transforming growth factor-β1 (TGF-β1) and improve the expression of collagen type Ⅲ . Therefore, the prescription of the extract A has better anti-photo-aging effect.