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目的探讨高功率微波(HPM)辐射对大鼠脑皮质和海马形态结构以及神经元神经肽Y (NPY)、神经型-氧化氮合酶(nNOS)表达的影响。方法分别采用10,30和100 mW/cm~2HPM辐射Wistar大鼠,并于微波辐射后6 h,1 d,3 d,7 d,14 d和28 d时处死,同时取出大脑皮质和海马组织。通过HE染色观察实验大鼠脑皮质及海马的形态结构改变情况;采用免疫组化和图像分析技术检测实验大鼠脑皮质和海马神经元NPY、nNOS表达的变化。结果实验大鼠经10,30,100 mW/cm~2HPM辐射后,其脑皮质及海马区神经元固缩、深染;HPM辐射后3 d时,大鼠神经元胞浆内nNOS表达显著增加(P<0.05),NPY表达显著降低(P<0.01)。结论10,30及100 mW/cm~2HPM辐射可引发大鼠脑皮质和海马神经元损伤,致使其NPY及nNOS表达异常。
Objective To investigate the effect of high power microwave (HPM) radiation on the morphological structure of cerebral cortex and hippocampus, neuropeptide Y (NPY) and neuronal nitric oxide synthase (nNOS) expression in rats. Methods Wistar rats were irradiated with 10, 30 and 100 mW / cm ~ 2HPM respectively, and were sacrificed at 6 h, 1 d, 3 d, 7 d, 14 d and 28 d after microwave irradiation. Cerebral cortex and hippocampus . The morphological changes of cerebral cortex and hippocampus were observed by HE staining. The expression of NPY and nNOS in the cortex and hippocampus were detected by immunohistochemistry and image analysis. Results The neurons in cerebral cortex and hippocampus were contracted and stained deeply after irradiated by 10, 30, 100 mW / cm ~ 2HPM. At 3 d after HPM irradiation, the expression of nNOS in neurons was significantly increased (P <0.05), NPY expression was significantly lower (P <0.01). Conclusion Radiation at 10, 30 and 100 mW / cm ~ 2HPM can induce neuronal damage in the cerebral cortex and hippocampus, resulting in abnormal expression of NPY and nNOS.