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目的 :研究人肺癌组织细胞NHE - 1基因表达水平 ;探讨人肺癌细胞在整体条件下PHi调节的分子机制及其生物学意义。方法 :以半定量逆转录PCR研究了 1 4例人肺癌组织手术新鲜标本的NHE - 1mRNA的表达水平。结果 :人肺癌组织细胞NHE - 1mRNA表达水平非常显著地高于正常肺组织 (p <0 0 0 1 ) ,系正常肺组织的 1 1 81倍。结论 :人肺癌组织细胞通过上调NHE - 1基因超表达 ,以增强Na+ /H+ 交换 ,可能是其在整体条件下PHi调节的主要分子机制和特点。
Objective: To study the expression level of NHE-1 gene in human lung cancer cells, and explore the molecular mechanism and biological significance of PHi regulation in human lung cancer cells under overall conditions. Methods : The expression level of NHE-1 mRNA in fresh specimens of 14 human lung cancer tissues was studied by semi-quantitative RT-PCR. Results: The expression level of NHE-1 mRNA in human lung cancer cells was significantly higher than that in normal lung tissues (p < 0 0 1), which was 1 1 81 times that of normal lung tissues. CONCLUSION: Human lung cancer cells up-regulate NHE-1 gene overexpression to enhance Na+/H+ exchange, which may be the main molecular mechanism and characteristics of PHi regulation under overall conditions.