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目的 探讨血管内皮生长因子 (VEGF)含量对非小细胞肺癌 (NSCLC)血道转移的影响。方法 外周血中细胞角蛋白 (CK)阳性细胞经磁性细胞分选技术分离后 ,采用细胞学、免疫组化及免疫荧光染色对其中的肺癌细胞进行特性分析。外周血肺癌细胞含量按已建立的流式细胞仪检测方法作定量分析。VEGF含量采用ELISA测定。结果 外周血肺癌细胞具有明显的核异型 ,表达端粒酶逆转录酶 (hTERT)及上皮细胞标志CK ,可被抗人NSCLC特异性单抗S5A10 2染色 ,但不表达白细胞抗原CD34及CD4 5。 15 4例NSCLC患者中 ,外周血肺癌细胞阳性 4 4例 ,阳性率为 2 8.6 %。癌细胞阳性率与病期进展相关。NSCLC患者血浆VEGF含量显著高于健康人及肺部良性疾患者。VEGF含量与肺腺癌的病期进展密切相关。在血循环癌细胞阳性NSCLC患者中 ,VEGF含量增高者 ,癌细胞数量显著高于VEGF不增高者。结论 NSCLC患者血浆VEGF明显增高 ,在血循环癌细胞阳性的患者中 ,VEGF高表达可促使癌细胞血道转移 ,表现为血循环癌细胞数量明显增加。
Objective To investigate the effect of vascular endothelial growth factor (VEGF) on the hematogenous metastasis of non-small cell lung cancer (NSCLC). Methods Cytokeratin (CK) positive cells in peripheral blood were separated by magnetic cell sorting technique. The characteristics of lung cancer cells were analyzed by cytology, immunohistochemistry and immunofluorescence staining. Lung cancer cells in peripheral blood flow cytometry has been established by quantitative detection methods for quantitative analysis. VEGF content was measured by ELISA. Results The peripheral blood of lung cancer cells showed obvious nuclear atypia. The expression of telomerase reverse transcriptase (hTERT) and epithelial cell marker CK was stained with anti-human NSCLC-specific monoclonal antibody S5A10 2 but not by leukocyte antigens CD34 and CD4 5. Of 154 NSCLC patients, 4 4 were positive for peripheral blood lung cancer cells, with a positive rate of 22.6%. The positive rate of cancer cells is related to the progress of the disease. Plasma VEGF levels in patients with NSCLC were significantly higher than those in healthy individuals and patients with benign lung diseases. VEGF content is closely related to the stage of progression of lung adenocarcinoma. In circulating cancer cells positive NSCLC patients, VEGF content increased, the number of cancer cells was significantly higher than those without VEGF. Conclusion The plasma VEGF in patients with NSCLC is significantly higher. In the patients with positive circulating blood cancer, high expression of VEGF can promote the metastasis of cancer cells, showing a marked increase in the number of circulating cancer cells.