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用BALB/C小鼠作为动物模型,应用放射免疫分析法对烧伤后早期小鼠腹腔液申的前列腺素E_2(PGE_2)进行动态测定,同时检测小鼠脾淋巴细胞对刀豆蛋白A刺激的反应性.结果表明,烧伤后7d内各期的PGE_2水平均明显高于正常对照值(67.2±36.8nmol/L,P<0.05～0.001);同时脾淋巴细胞对刀豆蛋白A刺激的反应性在烧伤后明显降低(P<0.05～0.001).体外实验也表明,烧伤鼠腹腔液提取物对正常鼠的淋巴细胞转化功能有抑制作用,与未烧伤鼠的腹腔液提取物的作用相比,具有明显差异(P<0.05).提示烧伤后体内的PGE_2生成明显增多,而且对淋巴细胞转化功能具有一定程度的抑制作用.说明烧伤后早期体内PGE_2的增高是介导免疫功能抑制的因素之一.
BALB / C mice were used as animal models. Radioimmunoassay was used to determine prostaglandin E_2 (PGE_2) in peritoneal fluid of early postburn mice. Meanwhile, splenic lymphocyte responses to concanavalin A stimulation The results showed that the levels of PGE_2 in each stage of 7d after burn were significantly higher than those in normal control group (67.2 ± 36.8nmol / L, P <0.05-0.001). Meanwhile, the reactivity of splenic lymphocytes to concanavalin A Burn significantly decreased (P <0.05 ~ 0.001) .In vitro experiments also showed that the rat peritoneal fluid extract on normal mice lymphocyte transformation function was inhibited, compared with non-burn rat peritoneal fluid extract, compared with the (P <0.05), suggesting that the PGE 2 production in vivo increased significantly after burn, but also inhibited the transformation of lymphocytes to a certain extent.It is indicated that the increase of PGE 2 in the early postburn phase is one of the factors that mediate the inhibition of immune function.