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目的:建立高效液相色谱法同时测定冠心丹参中三七皂苷R1、人参皂苷Rg1和人参皂苷Rb1含量的方法。方法:采用Kromasil-C18(250mm×4.6mm,5μm)色谱柱,以乙腈-水为流动相,梯度洗脱,流速1.0mL.min-1,柱温23℃,检测波长203nm。结果:三七皂苷R1、人参皂苷Rg1、人参皂苷Rb1进样量分别在0.0655～1.092μg/mL(r=0.9998),0.1980～3.2997μg/mL(r=0.9998),0.1802～3.0032μg/mL(r=0.9999)范围内与峰面积呈良好线性关系;平均回收率分别为98.8%,100.3%,100.4%,RSD均小于3%。结论:所建立的方法操作简便,测定结果准确,重复性好,可用于冠心丹参胶囊的质量控制。
Objective: To establish a method for simultaneous determination of notoginsenoside R1, ginsenoside Rg1 and ginsenoside Rb1 in Radix Salviae Miltiorrhizae with high performance liquid chromatography (HPLC). Methods: Kromasil-C18 (250 mm × 4.6 mm, 5 μm) was used as the mobile phase. The mobile phase consisted of acetonitrile and water with a gradient of 1.0 mL · min-1. The column temperature was 23 ℃ and the detection wavelength was 203 nm. Results: The injection volumes of notoginsenoside R1, ginsenoside Rg1 and ginsenoside Rb1 were 0.0655-1.092μg / mL (r = 0.9998), 0.1980-3.2997μg / mL (r = 0.9998), 0.1802-3.0032μg / mL r = 0.9999). The average recoveries were 98.8%, 100.3% and 100.4%, respectively. The RSDs were all less than 3%. Conclusion: The established method is simple, accurate and reproducible. It can be used for the quality control of Danshen Danshen capsule.