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目的 :研究皮质酮在ＰＣ1 2细胞是否能激活Ｅｒｋ1 /2ＭＡＰＫ及探讨其胞内信号转导机制。方法 :( 1 )ＰＣ1 2细胞培养 ;( 2 )免疫蛋白印迹 (Ｗｅｓｔｅｒｎｂｌｏｔｔｉｎｇ)法检测Ｅｒｋ1 /2ＭＡＰＫ的磷酸化 ;( 3)用间接免疫荧光染色法观察激活的Ｅｒｋ 1 /2ＭＡＰＫ由胞质
Objective: To investigate whether corticosterone can activate Erk1 / 2MAPK in PC1 2 cells and explore its intracellular signal transduction mechanism. Methods: (1) Cultured PC12 cells; (2) Erk1 / 2MAPK phosphorylation was detected by Western blotting; (3) The expression of activated Erk1 / 2MAPK was detected by indirect immunofluorescence staining