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1999年6月2日,青海省平安县洪水泉乡永安村发生因宰剥死骡而致炭疽1例,现报告如下:1 概况1999年5月下旬永安村农民许某饲养的骡子精神萎糜,排黑色大便,5月30日因呼吸困难窒息死亡.许某与本村村民6人参加死骡宰剥,骡肉分给40余人食用.6月1日许某右前臂掌侧出现一小疖肿,随即自行挤破,数小时后右前臂肿胀,呈暗红色,继之外围肿胀明显,触痛不明显,病灶中心区变黑,溃疡面直径2.5～3cm,患侧淋巴结肿大,在海东地区医院初步诊断为疑似传染病,6月2日省、县卫生防疫站专业人员在疫源地开展调查.2 实验室检查取许某病灶分泌物和溃疡面结痂物直接涂片镜检发现革兰氏染色阳性、两端平截、粗大杆菌.2.1 骡头皮组织培养 将研碎的组织作增菌培养,在PH8.4～8.6的碱性培养琼脂板上,可见菌落呈典型卷发状,经革兰氏染色,镜下见革兰氏染色阳性粗大、呈竹节链状排列的杆菌.
June 2, 1999, Ping’an County, Qinghai Province, Yongquan Village, flood springs occurred because of the exploitation of mule caused by anthrax in 1 case, are as follows: 1 Overview In late May 1999 farmers Yong Xu village reared Xu spirit mucus , Row black stool, May 30 due to respiratory distress choking death Xu and the village villagers participated in the death of six mule slaughter, Mulei to more than 40 people eat June 1 Xu forearm arm palm side appears a small 疖Swollen, then squeeze itself, a few hours after the right forearm swelling, dark red, followed by obvious swelling outside the obvious tenderness, lesion center area darker ulcer surface diameter 2.5 ~ 3cm, ipsilateral lymph nodes, in the sea Eastern Hospital was initially diagnosed as suspected infectious disease, provincial and county health and epidemic prevention stations on June 2 professionals in the epidemic investigation .2 laboratory tests allow a lesion secretion and ulcerated scab direct smear microscopy Gram staining was found, both ends of truncated, coarse bacilli.2.1 mule scalp tissue culture will be pulverized tissue enrichment culture, alkaline culture in the PH8.4 ~ 8.6 agar plate, we can see the typical colonies were curly hair , Gram stain, see the Gram stain positive gross, was arranged in bamboo chain Bacteria.