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氧化的低密度脂蛋白(LDL)在动脉粥样硬化过程中是一个重要因素,因此,其血循环浓度在冠心病患者中可能增高。本研究目的是观察严重冠心病患者的氧化脂蛋白是否高于无症状的健康对照者。病人和方法证实有心肌梗塞的冠心病患者23例,男21例,女2例,年龄44～75(平均60.8±1.8)岁。23例无症状对照组,男16例,女7例,年龄36～66(平均48.4±19)岁。两组均进行下列检测:脂蛋白分析,采用高效液相色谱法(HPLC)测定 LDL 及极低密度脂蛋白(VLDL)的氧化胆固醇(4-胆甾烯-3-酮和20α-羟基胆固醇)的含量,通过硫代巴比妥酸反应性物质(TBARS)方法测定丙二醛(MDA)的含量,用来评价脂质的过氧化作用以及分析赖氨酸的含量。
Oxidized low-density lipoprotein (LDL) is an important factor in the process of atherosclerosis, therefore, its blood circulation concentration may be increased in patients with coronary heart disease. The purpose of this study was to investigate whether the serum levels of oxidized lipoproteins in patients with severe coronary heart disease are higher than those in asymptomatic healthy controls. Twenty-three patients with coronary heart disease with myocardial infarction were confirmed by the patients and methods. There were 21 males and 2 females, aged 44-75 years (mean, 60.8 ± 1.8) years. 23 asymptomatic control group, 16 males and 7 females, aged 36 to 66 (mean 48.4 ± 19) years. The following tests were performed in both groups: lipoprotein analysis, determination of oxidized cholesterol (4-cholesten-3-one and 20α-hydroxycholesterol) of LDL and very low density lipoprotein (VLDL) by high performance liquid chromatography The content of malondialdehyde (MDA) was determined by thiobarbituric acid reactive substance (TBARS) method to evaluate the lipid peroxidation and analyze the content of lysine.