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近两年来,一种严重的传染性疾病在广西斑点叉尾(鱼回)养殖业中流行,造成鱼苗100%死亡;成鱼的损失也高达30%～80%不等。为了确定该病病原,本试验对患病斑点叉尾(鱼回)脑、肝脏、脾脏和肾脏组织进行了细菌的分离、鉴定及致病性试验。结果从南宁和合浦两地分离到NN和HP两株G短杆菌,人工感染试验表明,腹腔注射感染可引起健康斑点叉尾(鱼回)100%死亡,浸泡感染可引起30%～55%死亡,并且感染发病的斑点叉尾(鱼回)症状与自然发病症状相似。两株细菌的形态特征、培养特性和生理生化反应指标均与国外报道的(鱼回)爱德华氏菌参考菌株(JCM1680)相同;两株细菌的16S rRNA基因序列与JCM1680菌株16s rRNA基因序列同源性均为99.3%。以上研究证实,NN和HP两株细菌为致病性(鱼回)爱德华氏菌,其引起的传染性疾病为斑点又尾(鱼回)月而败血症。本研究首次对国内斑点叉尾(鱼回)肠败血症进行病原菌的分离和鉴定,证实了斑点又尾(鱼回)肠败血症在广西斑点叉尾(鱼回)养殖业中的流行及造成严重经济损失,对国内该病的控制及斑点叉尾(鱼回)健康养殖具有重要的指导意义。
In the past two years, a serious epidemic of infectious diseases in Guangxi Channel Catfish (fish back) aquaculture epidemic, resulting in 100% of fry death; adult fish losses as high as 30% to 80%. In order to determine the pathogen of the disease, the experiment isolated, identified and tested the pathogenic bacteria of diseased catfish, brain, liver, spleen and kidney. Results Two strains of N. gravis were isolated from Nanning and Hepu. The artificial infection test showed that intraperitoneal injection could cause 100% death of healthy channel catfish, and soaking infection could cause 30% -55% death , And the incidence of catfish (fish back) symptoms and symptoms of natural disease similar. The morphological characteristics, culture characteristics and physiological and biochemical indexes of the two strains of bacteria were the same as the reference strains (JCM1680) reported abroad. The 16S rRNA gene sequences of the two strains were homologous to the 16S rRNA gene of JCM1680 strain Sex are 99.3%. The above study confirmed that NN and HP two bacteria pathogenic (fish back) Edwardsiella, which caused the infectious disease for the spot and tail (fish back) month of sepsis. This study, for the first time, isolated and identified pathogenic bacteria from domestic catfish (FISH) enteric septicemia, confirming the prevalence of spotted and tailed (fish-back) enteric septicemia in Guangxi Channel Catfish (FISH) aquaculture and causing serious economic The loss of domestic control of the disease and channel catfish (fish back) healthy farming has an important guiding significance.