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目的观察携带颗粒溶素(Granulysin,GLS)活性肽基因的减毒沙门菌对小鼠黑色素瘤的治疗作用。方法将小鼠黑色素瘤细胞株B16接种C57 BL/6J小鼠,复制小鼠黑色素瘤模型。将pBudCE4.1-S9K重组沙门菌以不同剂量及不同时间接种于小鼠肿瘤内,检测肿瘤体积,确定最佳治疗剂量和起始时间。将模型小鼠随机分为PBS组、减毒沙门菌组、pBudCE4.1重组沙门菌组和pBudCE4.1-S9K重组沙门菌组,分别采用最佳治疗剂量,在最佳治疗起始时间开始治疗,每周1次,共3次。从成瘤开始,每2 d测量肿瘤的最长径(L)和最短径(S),计算肿瘤体积,绘制肿瘤生长曲线,并观察小鼠的荷瘤存活期。结果小鼠黑色素瘤模型复制成功。重组沙门菌的最佳治疗剂量和起始时间分别为107个/ml和第4天。与PBS组、减毒沙门菌组和pBud-CE4.1重组沙门菌组相比,pBudCE4.1-S9K重组沙门菌组能明显抑制模型小鼠肿瘤的生长,使肿瘤体积增长延缓,并明显延长荷瘤小鼠的存活期。结论 pBudCE4.1-S9K重组减毒沙门菌对小鼠黑色素瘤具有一定的治疗作用。
Objective To observe the therapeutic effect of attenuated Salmonella carrying Granulysin (GLS) active peptide gene on mouse melanoma. Methods Murine melanoma cell line B16 was inoculated into C57 BL / 6J mice to replicate the mouse melanoma model. The pBudCE4.1-S9K recombinant Salmonella were inoculated into the tumor of mice at different doses and times, and the tumor volume was measured to determine the optimal therapeutic dose and start time. The model mice were randomly divided into PBS group, attenuated Salmonella group, pBudCE4.1 recombinant Salmonella group and pBudCE4.1-S9K recombinant Salmonella group, respectively, the best treatment dose, treatment at the start of the best treatment , Once a week, a total of 3 times. From the beginning of tumor formation, the longest diameter (L) and the shortest diameter (S) of the tumor were measured every 2 days, the tumor volume was calculated, the tumor growth curve was drawn, and the tumor-bearing survival of the mice was observed. Results The mouse melanoma model was successfully replicated. The optimal therapeutic dose and initial time of recombinant Salmonella were 107 cells / ml and 4 days respectively. Compared with the PBS group, the attenuated Salmonella group and the pBud-CE4.1 recombinant Salmonella group, the pBudCE4.1-S9K recombinant Salmonella group could significantly inhibit the tumor growth of the model mice and delay the tumor volume growth and significantly prolonged The survival of tumor-bearing mice. Conclusion pBudCE4.1-S9K recombinant attenuated Salmonella has a certain therapeutic effect on mouse melanoma.