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目的:建立HPLC同时测定复方小艾飞中槲皮素、山柰素、乔松素、高良姜素4种黄酮类成分及胡椒碱含量的方法。方法:采用Hypersil BDS C18色谱柱(4.6 mm×250 mm,5μm),以乙睛(A)-1%磷酸溶液(B)-甲醇(C)为流动相,梯度洗脱(0~12 min,28%A∶52%B∶20%C;12~13 min,28%~30%A,52%~50%B,20%C;13~45 min,30%A∶50%B∶20%C),流速1.0 m L·min-1,柱温30℃,进样量20μL,检测波长280 nm。结果:槲皮素、山柰素、乔松素、高良姜素、胡椒碱的标准曲线分别为Y=357 314X-88 066(r=0.999 9),Y=442 853X-74 204(r=0.999 9),Y=647 926X-327 436(r=0.999 9),Y=471 672 X-256 408(r=0.999 9),Y=290 000X-391 906(r=0.999 9),线性关系良好。平均加样回收率(n=9)分别为98.4%(RSD 1.6%),98.9%(RSD 1.7%),101.3%(RSD 2.2%),98.6%(RSD 2.1%),98.6%(RSD 1.8%)。结论:该方法准确、简便,具有良好的重复性,可用于维吾尔族药复方小艾飞的质量控制。
Objective: To establish a method for the simultaneous determination of 4 flavonoids and piperine in Compound Astra? METHODS: Hypersil BDS C18 column (4.6 mm × 250 mm, 5 μm) was used with a mobile phase of acetonitrile (A) -1% phosphoric acid (B) -methanol (C) 28% A: 52% B: 20% C; 12-13 min, 28% -30% A, 52% -50% B, 20% C; 13-45 min, 30% A: 50% B: The flow rate was 1.0 m L · min-1. The column temperature was 30 ℃. The injection volume was 20 μL and the detection wavelength was 280 nm. Results: The standard curves of quercetin, kaempferol, jojoba, galangin and piperine were Y = 357 314X-88 066 (r = 0.999 9) and Y = 442 853X- 74 204 (r = 0.999 9) , Y = 647 926X-327 436 (r = 0.999 9), Y = 471 672 X-256 408 (r = 0.999 9) and Y = 290 000X-391 906 (r = 0.999 9). The average recovery was 98.4% (RSD 1.6%), 98.9% (RSD 1.7%), 101.3% (RSD 2.2%), 98.6% (RSD 2.1%) and 98.6% (RSD 1.8% ). Conclusion: This method is accurate, simple and reproducible. It can be used for the quality control of Xiaofei, a compound drug of Uygur.