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目的通过分析耐亚胺培南铜绿假单胞菌(PAE)整合子所携带的耐药基因,探讨其耐药机制,为临床用药、院内感染防控和新药研发提供参考。方法根据美国临床和实验室标准协会(CLSI)2015年标准,应用纸片扩散法进行药敏试验,采用多重聚合酶链式反应对临床分离的48株亚胺培南耐药铜绿假单胞菌进行整合酶基因(int)检测。结果药敏试验示48株亚胺培南耐药铜绿假单胞菌均为广泛耐株。5株为Ⅰ类整合酶阳性;24株为Ⅱ类整合酶阳性;6株Ⅰ、Ⅱ类整合酶均为阳性;未检出Ⅲ类整合酶。结论亚胺培南耐药铜绿假单胞菌大多具备Ⅰ、Ⅱ类整合酶基因,这是泛耐株的重要耐药机制。
OBJECTIVE: To analyze the resistance mechanism of imipenem-resistant Pseudomonas aeruginosa (PAE) integron and to provide a reference for clinical drug use, prevention and control of nosocomial infections and new drug development. Methods According to the standard of CLSI (2015) by American Society of Clinical and Laboratory Standards (CLSI), the susceptibility test was carried out by disk diffusion method, and 48 strains of imipenem-resistant Pseudomonas aeruginosa Integrase gene (int) test. Results Susceptibility test showed that 48 strains of imipenem-resistant Pseudomonas aeruginosa were widely resistant. 5 strains were positive for class I integrase; 24 strains were positive for class II integrase; 6 strains were positive for class I and class II integrants; and no class III integrase was detected. Conclusion Most of imipenem-resistant Pseudomonas aeruginosa possess class I and class II integrase genes, which is an important drug resistance mechanism of pan-resistant strains.