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目的:建立一种测定野艾蒿中总黄酮含量的方法。方法:以乙醇为溶剂,利用索氏提取器提取野艾蒿中总黄酮,以芦丁为对照品,用亚硝酸钠及硝酸铝在碱性溶液中显色,采用可见分光光度法,在510nm波长处测定吸光度。结果:野艾蒿中总黄酮的含量为21.82mg/g,加样回收率为98.8%。结论:该方法简便,结果准确,重现性好,可作为检验野艾蒿中总黄酮含量的一种手段,适用于野艾蒿药材的日常分析和质量控制。
Objective: To establish a method for determination of total flavonoids in Artemisia annua. Methods: The total flavonoids of Artemisia annua L. were extracted with ethanol as solvent and Soxhlet extractor. Rutin was used as reference substance, and developed with sodium nitrite and aluminum nitrate in alkaline solution. Visible spectrophotometry Absorbance measured at the wavelength. Results: The content of total flavonoids in Artemisia annua was 21.82mg / g, and the recovery rate was 98.8%. Conclusion: The method is simple, accurate and reproducible. It can be used as a means to test the content of total flavonoids in Artemisia annua. It is suitable for routine analysis and quality control of Artemisia ordosica.