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本文采用 MTT法观察螺旋藻粗粉及提取物对 CTL L细胞和正常 BALB/ c小鼠脾细胞分泌 IL - 2的影响。实验结果表明 ,所用的螺旋藻的 3个组份在实验浓度范围内对 CTLL细胞无增殖活化作用、螺旋藻粗粉及提取物多糖、藻兰蛋白 1、0 .1和 0 .0 1g· L-1浓度时均对正常小鼠脾细胞分泌 IL - 2有促进作用 ,藻兰蛋白部分作用最强 ,其它提取物作用次之 ,在上述浓度范围内 IL - 2分泌水平有剂量依赖关系。高浓度 (1g· L-1)粗粉及提取物具有协同 Con A(2 mg· L-1)刺激小鼠脾细胞分泌 IL - 2的作用
In this study, MTT assay was used to observe the effects of spirulina meal and extracts on the secretion of IL-2 from CTL L cells and normal BALB/c mouse spleen cells. The experimental results showed that the three components of Spirulina spp. used had no proliferative and activating effects on CTLL cells within the experimental concentration range, Spirulina coarse powder and extract polysaccharides, Phycobacterial protein 1, 0.1 and 0.01 g·L The concentration of IL-1 in the spleen cells of normal mice was promoted by the concentration of -1, and the most strong effect of phytoplankton was obtained. The effect of other extracts was the second, and the secretion of IL-2 was dose-dependent in the above concentration range. High-concentration (1g·L-1) Coarse Powder and Extracts Cooperate with Con A (2 mg·L-1) to Stimulate Secretion of IL-2 by Mouse Spleen Cells