,Simulation of Far-Field Superresolution Fluorescence Imaging with Two-Color One-Photon Excitation o

来源 :中国物理快报(英文版) | 被引量 : 0次 | 上传用户:choasy
下载到本地 , 更方便阅读
声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架
论文部分内容阅读
We propose to achieve far-field super-resolution imaging by using offset two-color one-photon(2C1P) excitation of reversible photoactivatable fluorescence proteins.Due to the distinctive photoswitching performance of the proteins,such as dronpa,the fluorescence emission will only come from the overlapped region of activation beam and excitation beam.The analysis solution of rate equation shows that the resolution of offset 2C1P microscope is "engineered" by laser power of excitation and activation beams and the power ratio between them.Superior lateral and transverse resolution is theoretically demonstrated compared with conventional fluorescence scanning microscopy.
其他文献
期刊
期刊
期刊
An efficient mid-wave infrared gain-switched Cr2+ :ZnSe laser pumped by a 2.058μm Tm,Ho:YVO4 laser is reported.As much as 3.86 W output is achieved with the pu
期刊
期刊
期刊
In order to obtain the influence of the luminance at photopic level on the neural mechanism,a neural contrast sensitivity function(NCSF) measurement system is e
期刊
期刊
期刊
A high-average-power diode-pumped narrowband regenerative chirped pulse amplifier is developed using the thin-rod Nd:YAG laser architecture for optical parametr
期刊