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对北青龙衣中总多糖进行分离纯化,获得分子量均一多糖,并对其进行结构分析。北青龙衣药材经沸水提取,浓缩醇沉,木瓜蛋白酶脱蛋白,D101大孔树脂柱纯化脱色,再经DEAE Cellulose 52离子交换柱层析、Sephacryl S-300凝胶柱层析得到纯化多糖组分。采用高效凝胶渗透色谱-蒸发光检测器对其进行纯度鉴定、分子量测定。气-质联用、红外光谱分析其单糖组成与结构。北青龙衣多糖经分离得到两个均一组分PJP-1a、PJP-3a,其分子量分别为:1.34×103Da、1.70×107Da。气-质联用分析PJP-3a是由鼠李糖、阿拉伯糖、木糖、甘露糖、葡萄糖和半乳糖组成,单糖摩尔比为:4.56∶7.53∶1∶2.48∶41.4∶17.94。红外光谱提示PJP-1a、PJP-3a可能均具有吡喃环结构,糖苷键的连接方式均为β型,PJP-1a存在乙酰氨基结构。
The total polysaccharide in Beilong green shirt was isolated and purified to obtain uniform molecular weight polysaccharide, and its structural analysis. North Qinglongyi herbs were extracted by boiling water, concentrated alcohol Shen, papain deproteinized, D101 macroporous resin column purification decolorization, and then by DEAE Cellulose 52 ion exchange column chromatography, Sephacryl S-300 gel column chromatography to obtain purified polysaccharide components . Purity identification and molecular weight determination were carried out by high performance gel permeation chromatography - evaporative light detector. GC-MS and IR spectroscopy to analyze its monosaccharide composition and structure. PJP-1a and PJP-3a were isolated from Beiqinglongyi polysaccharide, and their molecular weights were 1.34 × 103Da and 1.70 × 107Da, respectively. Gas-mass spectrometry PJP-3a is composed of rhamnose, arabinose, xylose, mannose, glucose and galactose, monosaccharide molar ratio: 4.56: 7.53: 1: 2.48: 41.4: 17.94. Infrared spectra suggest PJP-1a and PJP-3a may have pyran ring structure, the linkage of glycosidic bond is β-type, PJP-1a has acetamido structure.