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为探讨造血生长因子不同组合方式对脐血ＣＤ３４＋细胞的扩增作用、扩增细胞性能改变及合理的扩增时机，利用吸附单克隆抗体——磁珠分离系统分选出９５％～９９％纯度的脐血ＣＤ３４＋细胞，在体外液体培养体系中，经重组人干细胞因子（ｒｈＳＣＦ）、白细胞介素（ＩＬ）３、ＩＬ－６、粒－巨噬细胞集落刺激因子、粒细胞集落刺激因子（Ｇ－ＣＳＦ）和红细胞生成素（Ｅｐｏ）的不同组合扩增１～３周。结果表明，脐血ＣＤ３４＋细胞具有良好的扩增效应，细胞总数、集落形成细胞（ＣＦＣｓ）和ＣＤ３４＋细胞可分别扩增５００．０±８２．５倍、９４．０±２８．６倍和２４．０±３．８倍；ＳＣＦ是扩增最重要的生长因子，ＳＣＦ＋ＩＬ－３＋ＩＬ－６＋Ｇ－ＣＳＦ＋Ｅｐｏ组合的扩增效率最高；ＣＦＣｓ和ＣＤ３４＋细胞在１～２周维持在较高水平，以后即快速衰减；此外，扩增也加速了细胞的分化，至第３周时９３％ＣＦＣｓ为ＣＦＵ－ＧＭ，极少部分形成ＢＦＵ－Ｅ和ＣＦＵ－ＧＥＭＭ。因此，合理组合生长因子，把握适宜扩增时机，将有助于扩增细胞在质和量上满足移植和基因治疗等的要求。
To explore the effect of different combinations of hematopoietic growth factors on the expansion of umbilical cord blood CD34 + cells, the change of cell performance and the reasonable timing of amplification, the purity of 95% ~ 99% was determined by using the adsorption monoclonal antibody - magnetic bead separation system Of cord blood CD34 + cells were cultured in liquid culture system in vitro. Recombinant human stem cell factor (rhSCF), interleukin (IL) 3, IL-6, granulocyte-macrophage colony stimulating factor, -CSF) and erythropoietin (Epo) for 1 to 3 weeks. The results showed that the cord blood CD34 + cells had a good amplification effect. The total number of cells, colony forming cells (CFCs) and CD34 + cells were amplified by 500.0 ± 82.5 fold, 94.0 ± 28.6 fold and 24 respectively. 0 ± 3.8 times; SCF is the most important growth factor of amplification, the highest efficiency of SCF + IL-3 + IL-6 + G-CSF + Epo combination; CFCs and CD34 + cells in 1 to 2 weeks to maintain a high level, then rapid decay In addition, expansion also accelerated cell differentiation, with 93% of CFCs as CFU-GM by week 3, with very few forms of BFU-E and CFU-GEMM. Therefore, a reasonable combination of growth factors, to grasp the appropriate timing of amplification, will help to expand the cells in terms of quality and quantity to meet the requirements of transplantation and gene therapy.