论文部分内容阅读
以文蛤(Meretrix meretrix)和四角蛤蜊(Mactra veneriformis)为实验对象,将其暴露于石油烃浓度分别为0.1 mg/L,0.3 mg/L,1.0 mg/L和3.0 mg/L的海水中,并于实验开始后的0、0.5 d、1 d、3 d、6 d、10 d和15 d取样,并测定其消化盲囊和鳃丝谷胱甘肽硫转移酶(GST)和超氧化物歧化酶(SOD)活性。实验结果显示,两种蛤各组织GST和SOD酶活性在0.1 mg/L和0.3 mg/L处理组均明显高于对照组水平(P<0.05);除四角蛤蜊鳃丝GST酶活性一直被抑制外,其余两种蛤各组织酶活性在1.0 mg/L处理组都呈现先升高后被抑制的趋势;除文蛤消化盲囊SOD活性在暴露实验开始升高外,其余组织酶活性在3.0 mg/L处理组一直被抑制(P<0.05)。通过比较发现四角蛤蜊GST和SOD酶活性升高率在0.1 mg/L和0.3mg/L浓度组实验前期高于文蛤,但是在后期低于文蛤,在1.0 mg/L和3.0 mg/L浓度组四角蛤蜊酶活性抑制率明显高于文蛤(P<0.05)。结果表明,石油烃暴露条件下四角蛤蜊较文蛤表现更敏感,可以优先作为石油烃污染指示生物,鳃丝各指标的变化较消化盲囊明显,可以优先作为指示组织;GST和SOD酶的变化趋势具有一定一致性,以后相关研究和实践中可以将这两种指标结合测定以评价石油烃及其它有机物污染带来的风险。
Meretrix meretrix and Mactra veneriformis were exposed to seawater with petroleum hydrocarbon concentrations of 0.1 mg / L, 0.3 mg / L, 1.0 mg / L and 3.0 mg / L, respectively. Samples were taken at 0, 0.5 d, 1 d, 3 d, 6 d, 10 d and 15 d after the start of the experiment, and their digestive cyst and gill glutathione S-transferase (GST) and superoxide dismutase Enzyme (SOD) activity. The results showed that the activities of GST and SOD in the two clam tissues were significantly higher than those in the control group (P <0.05) at 0.1 mg / L and 0.3 mg / L, respectively. GST activity in the gill filaments of Clam clam The activities of other enzymes in the two clams increased first and then decreased, while the activities of other enzymes in the digestive capsules were 3.0 mg / L treatment group has been inhibited (P <0.05). The results showed that the increase rate of GST and SOD activity in clam clam was higher than that of clam in the concentrations of 0.1 mg / L and 0.3 mg / L in the early stage of the experiment, but lower than that of the clam in the late stage and 1.0 mg / L and 3.0 mg / L The inhibitory rate of clam antler activity was significantly higher than that of clam (P <0.05). The results showed that clam clam was more sensitive to clam than the clam under petroleum hydrocarbon exposure condition, which could be taken as the indicator of petroleum hydrocarbon pollution preferentially. The change of each index of gill filaments was more obvious than that of digestive blind capsule, Have some consistency, after relevant research and practice can combine these two indicators to determine the risk of petroleum hydrocarbon and other organic pollution.