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目的：阐明c－cyc表达在肾病发生、发展中的作用及其临床意义。方法：利用免疫组化ABC法结合微波抗原修复和流式细胞技术，对c－myc蛋白在52例肾癌组织及14例癌旁正常肾组织中的表达，进行了定性、定量的测定。结果：肾癌中c－myc蛋白的阳性率和阳性细胞率为76．92％和65．45％±29．19％，高于正常肾的14．29％和8．59％±3．66％（P＜0．005）。c－myc在肾癌中55．77％分布于胞浆，21．15％分布于胞核，在正常肾全部分布于胞核。其胸浆表达在病理分级G2G3级高于G1级（χ＝9．244，P＜0．05）；在临床分期T3、T4期高于T1、T2期（P＜0．01）。结论：c－myc的胞浆表达促进肾癌的发生、发展，并与恶性程度、临床分期有关，是预后不良的重要指征。
Objective: To elucidate the role of c-cyc expression in the pathogenesis and development of nephropathy and its clinical significance. METHODS: Using immunohistochemical ABC method combined with microwave antigen retrieval and flow cytometry, the expression of c-myc protein in 52 cases of renal cell carcinoma and 14 cases of normal renal tissue was determined qualitatively and quantitatively. Results: The positive rate and positive rate of c-myc protein in renal cell carcinoma were 76.92% and 65.45%±29.19%, higher than those in normal kidney 14.29% and 8.59%±3.66, respectively. % (P<0.005). 55.77% of c-myc is distributed in the cytoplasm in kidney cancer, 21.15% in the nucleus and all in the normal kidney. The thoracic pulp was expressed in pathological grade G2G3 grade higher than G1 grade (χ=9.244, P<0.05); in clinical stage T3, T4 was higher than T1, T2 grade (P<0.01). Conclusion: The cytoplasmic expression of c-myc promotes the occurrence and development of renal cancer, and is related to the degree of malignancy and clinical stage. It is an important indicator of poor prognosis.