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本研究以番茄(Lycopersicon esculentumM ill.)栽培品种“中杂9号”、“毛粉802”和“合作908”为试材,通过对番茄子叶、下胚轴的离体培养,研究不同基因型以及不同激素配比对植株再生的影响。结果表明:以下胚轴为外植体时3种材料均可诱导再生植株,其中中杂9号和毛粉802最适宜诱导愈伤组织和芽分化的培养基为MS+6-BA2.0mg/L+IAA0.5mg/L,合作908最适宜诱导愈伤组织和芽分化的培养基为MS+6-BA2.0mg/L+IAA0.4 mg/L。以子叶为外植体时毛粉802和合作908均可诱导出再生植株,其中毛粉802诱导愈伤组织及芽分化的最适宜培养基为MS+6-BA1.0mg/L+IAA0.2mg/L和MS+6-BA1.0mg/L+IAA0.5mg/L,合作908诱导愈伤组织及芽分化的培养基为MS+6-BA0.5mg/L+IBA0.5mg/L。两种外植体适宜的生根培养基均为MS+IAA0.5 mg/L。
In this study, tomato (Lycopersicon esculentum Mill.) Cultivars “Zhongza 9”, “Fufen 802” and “Cooperative 908” were used as materials to study the effects of different genotypes on the in vitro culture of tomato cotyledons and hypocotyls And the effect of different hormone ratio on plant regeneration. The results showed that all the three materials could induce regenerated plants with the following hypocotyls as explants. Among them, Zhongza 9 and WT 802 were the best medium for inducing callus and bud differentiation with MS + 6-BA 2.0 mg / L + IAA0.5mg / L, and the best 908 medium for inducing callus and bud differentiation was MS + 6-BA2.0mg / L + IAA0.4mg / L. When cotyledon explants were used as explants, the reconstituted plants could be induced by the co-cultivating 802 and the co-cultivating 908 respectively. The most suitable medium for inducing the callus and bud differentiation of the cole with 802 was MS + 6-BA1.0mg / L + IAA0.2mg / L and MS + 6-BA1.0mg / L + IAA0.5mg / L. The medium for the combination of 908 induction of callus and bud differentiation was MS + 6-BA0.5mg / L + IBA0.5mg / L. The appropriate rooting medium for both explants was MS + IAA 0.5 mg / L.