HPLC-DAD法同时测定木蝴蝶中4种化学成分的含量

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建立了HPLC-DAD法同时测定木蝴蝶中4种化学成分的含量.采用Agilent Eclipse XDB-C18色谱柱(4.6 mm×250 mm,5μm),以乙腈-0.1%冰醋酸水溶液进行梯度洗脱,流速:1 mL/min,柱温:30℃,检测波长:320 nm,进样量10 μL.结果表明:芦丁、木犀草苷、槲皮素、黄芩苷4个化合物在测定的范围内均呈现出良好的线性关系(r≥0.9995),回收率为94.0% ~ 98.0%.该方法操作简便,分离效果较好,灵敏度高,重现性良好,为较全面控制木蝴蝶药材的质
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