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目的探讨米力农对在体肺缺血再灌注(I/R)损伤的保护作用。方法夹闭大鼠左肺门1h,再灌注3h,建立在体缺血再灌注模型。将20只SD大鼠随机分成缺血再灌注组(对照组);米力农组(实验组)。米力农组缺血前30min给予颈静脉推注米力农1mg/kg。再灌注3h后摘取左肺测超氧化物歧化酶(SOD)活力、丙二醛(MDA)含量及髓过氧化物酶(MPO)活力并行病理学检查。结果再灌注3h后,两组间MPO活力无显著性差异。对照组MDA(nmol/mgprot)含量为0.88±0.06,显著高于实验组0.49±0.11(P<0.05),而SOD(U/mgprot)活力为16.09±10.73,显著低于实验组35.93±13.50。结论米力农对肺缺血再灌注损伤具有保护作用,可能与其抗氧化作用有关。
Objective To investigate the protective effect of Milrinone against lung injury following ischemia / reperfusion (I / R) injury in rats. Methods The left hilar of rats was occluded for 1 hour and then reperfused for 3 hours to establish a model of ischemia-reperfusion in vivo. Twenty SD rats were randomly divided into ischemia-reperfusion group (control group) and Milian group (experimental group). Milrinone 30min before giving the jugular vein injection of Milrinone 1mg / kg. After 3h of reperfusion, the activity of left lung SOD, the content of malondialdehyde (MDA) and the activity of myeloperoxidase (MPO) were detected. Results After 3h of reperfusion, there was no significant difference in MPO activity between the two groups. The content of MDA (nmol / mgprot) in the control group was 0.88 ± 0.06, which was significantly higher than that in the experimental group (0.49 ± 0.11, P <0.05), while the activity of SOD (U / mgprot) was 16.09 ± 10.73, significantly lower than that of the experimental group (35.93 ± 13.50). Conclusion Milrinone has a protective effect on lung ischemia-reperfusion injury, which may be related to its antioxidative effect.