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目的:探讨自噬相关基因宫颈鳞癌组织中微管相关蛋白1轻链3(LC3)的蛋白表达及与肿瘤发生、发展的关系。方法:采用免疫组化的方法检测122例宫颈鳞状细胞癌,35例宫颈上皮内瘤变(CIN)Ⅱ～Ⅲ,31例正常宫颈组织LC3蛋白的表达。结果:宫颈鳞癌、CIN组织和正常宫颈组织组织中LC3阳性率分别为51.6%、71.4%和83.9%,3组中LC3阳性表达呈下降趋势,χ2=20.837,P=0.000。LC3与肿瘤分化(χ2=16.678,P=0.000)、复发(χ2=4.817,P=0.028)相关。鳞状细胞癌抗原(SccAg,P=0.042)、肿瘤分化(P=0.010)、浸润深度(P=0.048)、淋巴结转移(P=0.001)、手术切缘(P=0.015)和LC3(P=0.025)表达是无进展生存时间(PFS)的危险因素;肿瘤分化(P=0.025)、淋巴结转移(P=0.001)是影响总生存时间(OS)的危险因素。多因素分析显示,淋巴结转移是PFS(P=0.015)和OS(P=0.002)的独立预后因素。结论:宫颈鳞癌组织LC3表达低于正常宫颈和CIN组织的表达,提示LC3在宫颈癌的发生、发展中可能起一定作用。
Objective: To investigate the protein expression of microtubule-associated protein 1 light chain 3 (LC3) and its relationship with tumorigenesis and progression in cervical squamous cell carcinoma of the autophagy. Methods: The expressions of LC3 protein in 122 cases of cervical squamous cell carcinoma, 35 cases of cervical intraepithelial neoplasia (CIN) Ⅱ ~ Ⅲ and 31 cases of normal cervical tissue were detected by immunohistochemistry. Results: The positive rates of LC3 in cervical squamous cell carcinoma, cervical intraepithelial neoplasia and normal cervical tissues were 51.6%, 71.4% and 83.9%, respectively. The positive expression of LC3 in the three groups showed a decreasing trend, χ2 = 20.837, P = 0.000. LC3 was correlated with tumor differentiation (χ2 = 16.678, P = 0.000) and recurrence (χ2 = 4.817, P = 0.028). (P = 0.010), depth of invasion (P = 0.048), lymph node metastasis (P = 0.001), surgical margin (P = 0.015) and LC3 (P = 0.025) were risk factors for progression-free survival time (PFS); tumor differentiation (P = 0.025) and lymph node metastasis (P = 0.001) were the risk factors influencing overall survival time (OS) Multivariate analysis showed that lymph node metastasis was an independent prognostic factor for PFS (P = 0.015) and OS (P = 0.002). Conclusion: The expression of LC3 in cervical squamous cell carcinoma is lower than that in normal cervical and CIN tissues, suggesting that LC3 may play a role in the occurrence and development of cervical cancer.