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目的比较腹腔镜手术和传统根治手术后大肠癌外周血VEGF-C活性的变化,探讨手术方式对VEGF-C活性表达的影响。方法利用酶联免疫吸附定量方法(ELISA)检测和比较两种手术前后大肠癌患者外周血VEGF-C活性。结果两组术前外周血的VEGF-C吸光度(OD)为0.537±0.026,术后传统根治手术为0.441±0.023;腹腔镜手术为0.411±0.021,圴较术前明显降低(P<0.01),但两者术后相比,差异无显著性(P>0.001)。结论大肠癌根治手术是降低患者外周血VEGF-C活性的主要因素,但手术方式对VEGF-C活性不构成重要影响。
Objective To compare the changes of VEGF-C activity in peripheral blood of patients with colorectal cancer after laparoscopic surgery and traditional radical surgery and to explore the effect of surgical modality on the expression of VEGF-C. Methods Enzyme-linked immunosorbent assay (ELISA) was used to detect and compare VEGF-C activity in peripheral blood of patients with colorectal cancer before and after operation. Results The preoperative peripheral blood VEGF-C absorbance (OD) was 0.537 ± 0.026, the postoperative traditional radical operation was 0.441 ± 0.023; the laparoscopic operation was 0.411 ± 0.021, significantly lower than that before operation (P <0.01) However, there was no significant difference between the two groups (P> 0.001). Conclusions Radical surgery of colorectal cancer is the main factor to reduce the activity of VEGF-C in peripheral blood of patients. However, the operation mode does not have an important influence on the activity of VEGF-C.