由于产生重组体DNA的技术,有可能制得RNA病毒基因组的DNA复制品,已使对RNA病毒的研究有了革命性的变化,这样制得的DNA复制品就可用来进行限制性内切酶分析与序列分析。因而可以获得对RNA病毒基因组结构方面研究的详细资料。本文作者采用了重组体DNA方法对脊髓灰质炎病毒和逆转录病毒的基因组进行了研究。为了制备一种脊髓灰白质炎病毒RNA基因组的DNA复制品,作者利用寡聚脱氧胸苷酸作引物,逆转录RNA为一负链DNA,再让此单链DNA折回 Due to the technique of producing recombinant DNA, it is possible to make a DNA replica of the RNA virus genome that has revolutionized the study of RNA viruses so that the DNA replicas thus prepared can be used for restriction enzyme Analysis and Sequence Analysis. Thus, detailed information on the genome structure of RNA viruses can be obtained. The authors used recombinant DNA methods to study the genomes of polioviruses and retroviruses. In order to prepare a DNA copy of the RNA genome of the myelo-parvovirus, the authors used oligodeoxythymidine as a primer, reverse transcribed RNA as a minus-strand DNA, and then the single-stranded DNA was folded back