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成年 Lewis 鼠(9-12周大)的视网膜胶质细胞用酶消化和梯度离心的方法分离和提纯。这些细胞有形态和胶质原纤维酸性蛋白(GFAP)的免疫化学着色的特征并伴有特殊的单克隆抗体。用纯细胞计数和氚标志的胸腺嘧啶渗合的测量时,其在培养中的增生是依赖于刀豆球蛋白-A 刺激脾脏单核的细胞上清液的加入到培养基而定。用已确定的 S-抗原和 PPD 特殊的 T-淋巴细胞与已存在的抗原和经辐照的胸腺细胞(2000-3000拉德)作为抗原递送细胞共同培养这些胶质细胞,产生有意义的抑制这些淋巴细胞的母细胞转化。我们相信,由活性淋巴细胞产物所致的视网膜胶质细胞的增殖与眼发炎时视网膜外膜的发展有关。另一方面,此资料证明随后这些胶质细胞能抑制淋巴细胞的活性。这种反馈抑制与眼发炎过程中的局部调节有关。
Retinal glia of adult Lewis rats (9-12 weeks old) were isolated and purified by enzymatic digestion and gradient centrifugation. These cells are characterized by immunochemical staining of the morphology and glial fibrillary acidic protein (GFAP) accompanied by specific monoclonal antibodies. When measured by pure cell count and tritiated thymidine incorporation, its proliferation in culture is dependent on the addition of cell supernatant of concanavalin-A stimulated spleen mononuclear cells to the medium. These glial cells were co-cultured with established S-antigen and PPD-specific T-lymphocytes with pre-existing antigen and irradiated thymocytes (2000-3000 rad) as antigen-delivered cells resulting in significant inhibition The mother cells of these lymphocytes are transformed. We believe that the proliferation of retinal glial cells caused by reactive lymphocyte products is associated with the development of the outer retinal membrane during ocular inflammation. On the other hand, this data demonstrates that these glial cells can subsequently inhibit lymphocyte activity. This feedback inhibition is related to the local regulation of ocular inflammation.