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该研究应用454 GS FLX Titanium高通量测序技术对5年生草麻黄Ephedra sinica的草质茎进行转录组测序,共获得48 389条表达序列标签(express sequence tags,ESTs),序列平均长度为373 bp。所得序列与Gen Bank中麻黄的EST合并拼接,获得18 801条一致性序列(unigene)。通过与公共数据库中的序列进行同源性比较分析对所得转录本进行功能注释,结果表明其中56.0%(10 531条)的unigenes与其他生物的已知基因具有一定程度的同源性。进一步分析获得了19条可能参与麻黄生物碱生物合成的序列(共编码9个关键酶),97条细胞色素P450序列,以及大量转录因子序列。该研究为麻黄生物碱类化合物的生物合成研究奠定了基础,同时为麻黄转录组学的研究提供了海量数据,对于麻黄的功能基因组学研究具有重要意义。
In this study, we used 454 GS FLX Titanium high-throughput sequencing technology to transcript the grass stems of 5-year-old Ephedra sinica to obtain 48 389 express sequence tags (ESTs) with an average length of 373 bp . The resulting sequence was pooled with Ephedra EST in Gen Bank to obtain 18 801 unigene. Functional annotation of the resulting transcripts by comparison with sequences in public databases revealed that 56.0% (10 531) of unigenes had some degree of homology with known genes from other organisms. A total of 19 possible sequences involved in biosynthesis of ephedra alkaloids (9 key enzymes), 97 cytochrome P450 sequences and a large number of transcription factors were obtained. This study lays the foundation for the biosynthesis research of ephedrine alkaloids and provides massive data for the study of ephedra transcriptomics, which is of great significance for the study of functional genomics of ephedra.