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本文报告了肿瘤坏死因子（ＴＮＦ）在内毒素休克中的作用。雄性Ｗｉｓｔａｒ大鼠静脉注射大肠杆菌内毒素（ＥｃｏｌｉＯ１１１Ｂ４，５ｍｇ·ｋｇ－１，）复制内毒素休克模型。应用Ｎｏｒｔｈｅｒｎ印迹杂交技术分析肝脏组织ＴＮＦ－ｍＲＮＡ表达水平，放免法测定血浆ＴＮＦ含量。结果发现内毒素攻击后２ｈ血浆ＴＮＦ含量明显升高（Ｐ＜００１）；肝脏组织ＴＮＦ－ｍＲＮＡ表达明显增加；内毒素攻击后２ｈ给予塞地塞米松（Ｄｅｘ，５ｍｇ·ｋｇ－１）、前列腺素Ｅ２（ＰＧＥ２，２ｍｇ·ｋｇ－１）均能抑制肝脏细胞ＴＮＦ－ｍＲＮＡＦ表达，降低血浆ＴＮＦ含量，提高休克大鼠的ＭＡＢＰ及２４ｈ存活率，具有明显的抗内毒素休克作用。研究结果表明ＴＮＦ在内毒素休克发病中具有重要的作用。
This article reports the role of tumor necrosis factor (TNF) in endotoxic shock. Male Wistar rats were injected with E.coli endotoxin (EcoliO111B4, 5 mg · kg-1, ip) to replicate the endotoxic shock model. The expression of TNF-mRNA in liver tissue was analyzed by Northern blot hybridization and the content of TNF in plasma was determined by radioimmunoassay. The results showed that the level of TNF was significantly increased 2 h after endotoxin challenge (P <001), and the expression of TNF-mRNA in liver tissue was significantly increased. Dex (5 mg · kg -1) Prostaglandin E2 (PGE2, 2 mg · kg-1) could inhibit the expression of TNF-mRNA in liver cells, decrease the content of TNF in plasma and increase the MABP and 24h survival rate in shock rats with obvious anti-endotoxic shock effect. The results show that TNF plays an important role in the pathogenesis of endotoxic shock.