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金黄色葡萄球菌(简称金葡菌)剥脱性毒素与临床上烫伤样皮肤综合征有关,婴儿对这种金葡菌化脓征特别易感。已发现在金葡菌噬菌体Ⅱ组菌株内有2种不同血清型的剥脱性毒素:ET-A,ET-B。编码ET-A的基因在细菌的染色体上,而编码ET-B的基因则位于27～29×10~6道尔顿的质粒中。该质粒同时还携带产细菌素R_1(BacR_1)的基因。产细菌素R_1的细菌对细菌素R_1不敏感,可根据此特性进行筛选。作者用具有ET-B质粒菌株与无该质粒的菌株在硝酸纤维素膜上作混合培养,发现ET-B质粒可经噬菌体转导,提示这种混合培养转导可能是机体内致病基因转移的一种机理。例如,体表正常菌群葡萄球菌可能作
Staphylococcus aureus (Staphylococcus aureus) exfoliative toxins and clinical scalded skin syndrome, the baby is particularly susceptible to the staphylococcus aureus. It has been found that there are 2 exfoliative toxins of different serotypes within the Staphylococcus aureus phage Group II strain: ET-A, ET-B. The gene encoding ET-A is on the chromosome of bacteria, whereas the gene encoding ET-B is located in a plasmid of 27-29x10-6 Daltons. The plasmid also carries the bacteriocin R_1 (BacR_1) gene. The bacteria producing bacteriocin R_1 are insensitive to bacteriocin R_1 and can be screened according to this characteristic. The authors used ET-B plasmid strains and strains without the plasmid mixed culture on nitrocellulose membrane and found that ET-B plasmid can be transduced by phage, suggesting that this mixed culture transduction may be the body pathogenic gene transfer A mechanism. For example, normal body surface Staphylococcus aureus may be made