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目的肿瘤微环境或者肿瘤细胞本身对免疫细胞的分化具有定向诱导作用,本研究目的在于了解胶质瘤细胞株U87MG外泌分子对Na觙ve CD4细胞分化可能的影响。方法外周血PBMC中分离Na觙ve CD4细胞,U87MG培养液上清以1/5量加至Na觙veCD4细胞培养体系中,培养7 d,检测Na觙ve CD4细胞分化增殖情况以及分化为Th1、Th2、Th17、Treg四个亚群量的变化。结果加有1/5量U87MG培养上清的Na觙ve CD4细胞分化成Th2减低,较对照组有显著性差异8.17%±2.08%vs 9.63%±2.48%(P<0.05),Th1较对照组均值低,但无显著性差异,Th17、Treg细胞在2组之间无明显差异。结论观察到U87MG外泌分子Na觙ve CD4分化为Th2细胞能力减弱,同时初步观察了Na觙ve CD4细胞分化为其他亚群的变化规律,为理解肿瘤细胞对Na觙ve CD4细胞分化影响提供了实验基础。
The purpose of the tumor microenvironment or tumor cells itself has a directional induction of immune cell differentiation, the purpose of this study is to understand the glioma cell line U87MG excretory molecules Na 觙 ve CD4 cells may affect the differentiation. Methods Na 觙 ve CD4 cells were isolated from peripheral blood PBMCs. The supernatant of U87MG medium was added to Na 觙 veCD4 cell culture system and cultured for 7 days. The differentiation and proliferation of Na 觙 ve CD4 cells and differentiation into Th1, Th2, Th17, Treg four subpopulations changes. Results Compared with control group, the percentage of Th2 cells in Na 觙 ve CD4 cells treated with U87MG culture supernatant was decreased by 8.17% ± 2.08% vs 9.63% ± 2.48% (P <0.05) Mean, but no significant difference, Th17, Treg cells in the two groups no significant difference. Conclusions It is observed that the differentiation of Na 觙 ve CD4 into Th2 cells in U87MG excretion is diminished. At the same time, the change of Na 觙 ve CD4 cells differentiation into other subpopulations is observed. To understand the influence of tumor cells on the differentiation of Na 觙 ve CD4 cells Experimental basis.